Ambrocio was last seen by her ex-husband on November 19, 1986. They had an argument and he dropped her off at the Church's Fried Chicken restaurant on Memorial Boulevard in Lakeland, Florida. She has never been heard from again. Her sister reported her missing.

Ambrocio left behind two children when she vanished. She was having marital problems at the time of her disappearance, but had not given any indication that she planned to leave. Authorities believe she met with foul play, but little evidence is available in her case.