Holt was last seen at the Howard Johnson's motel in Waldorf, Maryland on April 25, 1996. She called her estranged husband that day and made plans to meet him, but never showed up. She has never been heard from again. Holt's mother reported her as a missing person on June 12, 1996, approximately eight weeks after she disappeared.

Holt had acquaintances who were involved in drugs, and she was reportedly afraid of these individuals. At the suggestion of her mother, she had gone to live with a relative in Florida, but she returned to Maryland without her relatives' knowledge. Holt left behind two children. She had dropped out of sight for short periods before, but never for this long. Her husband isn't considered a suspect in her disappearance. Authorities suspect that foul play was involved in Holt's case.