Pospisil was last seen at a friend's home in the 100 black of 12th Street southeast on late Sunday, June 3, 2001. She had gone to the friend's at 8:45 p.m. to accompany her to the movie theater but the friend was not home. A two-door dark-colored Chrevolet Impala or Chevrolet Cavalier, early 1990's model, with dark tinted rear windows, pulled up the curb of Pospisil's friend's house. She spoke to the occupants for a short time, then got into the backseat of the car, saying she would get a ride with them. Pospisil has never been heard from again.

The Chevrolet was unfamiliar to Pospisil's friends and family but an acquaintance who witnessed her getting into it said she seemed to know the people inside. Police therefore classified her case as a runaway, but Pospisil's family believes she may have been taken against her will and may be in danger. She did not take her day-timer, makeup case, or any other personal belongings with her, and none of her friends have heard from her since she disappeared. Her loved ones say this is very uncharacteristic of her.

The investigation into Pospisil's disappearance leans towards the theory that gang members in the areas of Cedar Rapids and an area of Chicago, Illinois called "ghost town" may have involved in her case. She may have been seen in the spring of 2002 at a convenience store in Cedar Rapids, in the backseat of a car with Illinois license plates. The witness knows Pospisil well and is sure the girl in the vehicle was her. Police, when called, were unable to locate her or the vehicle, however.

Pospisil has relatives in Nevada but none of them have heard from her. She lived with her father, stepmother and three siblings when she disappeared, and was a freshman at Metro High School. Her school attendance was spotty. Pospisil's case remains unsolved. Many agencies classify her as an endangered runaway.